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成像极限

基于前沿染料工具、光学显微镜和单分子荧光解析算法开发，推进活细胞分子定位测量时空极限。部分典型结果如下表所示：

Living-cell targets Labeling strategy Precision
(Spatial resolution; nm)		 Priority
(spatial, temporal, applicational)		 Source
Lysosomes
  • Small molecule
  • Bioorthogonal
 13.9 (37.6)
~22
  • No wash, Background-free
  • 4D imaging (x, y, z, t)
  • 2.0 s (Temporal resolution)
  • ≥ 0-60 s time scale for imaging
  • Fusion and fission analysis
 Anal. Chem., 2022, 94, 7990
JACS, 2019, 141, 14491
Chin. Chem. Lett., 2021, 32, 3862
Chem. Commun., 2018, 54, 2842
RSC Adv., 2014, 4, 37547
Mitochondria
  • Small molecule (cristae)
  • TOMM20 (Halo-tag, outer membrane)
  • Bioorthogonal (cristae)
 ~25 (~50)
16.8 (110.5)
13.1 (94.4)
  • 2.5 s temporal resolution
  • 0-50 s time scale for imaging
  • Fusion and fission analysis
  • Resolving ferroptosis circularity
 JACS, 2019, 141, 6527
JACS, 2023, 145, 5125
Anal. Chem., 2018, 90, 2164
Anal. Chem., 2021, 93, 7833
Chin. Chem. Lett., 2021, 32, 3862
Endoplasmic reticulum (ER)
  • Sec61β (Halo-tag)
  • Retention peptide (SNAP-tag)
 17.3 (103.5)
27-29 (56.2)
  • 7.5 s temporal resolution
  • 0-60 s time scale for imaging
  • ER sheet dynamics visualization
  • Tension during inflammation
 JACS, 2023, 145, 5125
Biosens. Bioelectron., 2021, 189, 113378
Microtubules
  • β-Tubulin (Halo-tag)
  • α-Tubulin (Fixed cells)
 14.9 (101.8)
10.9 (121.8, 96.3)
  • Resolving microtubules filaments
  • Structural protein distribution
 JACS, 2023, 145, 5125
Anal. Chem., 2021, 93, 7833
Nucleus
  • Small molecule (DNA)
  • H2B (Halo-tag)
  • Bioorthogonal (H2B)
 ~21
20-22, 28.6 (178.8)
13.1 (94.4)
  • Resolving mitosis DNA distribution
 JACS, 2019, 141, 14491
Anal. Chem., 2021, 93, 7833
Chem. Commun., 2019, 55, 1951
JACS, 2023, 145, 5125
JACS, 2019, 141, 6527
Plasma membrane
  • Small molecule (PM)
 20.9, 23.2(68.5)
  • 3D imaging (x, y, z)
  • High-throughput
  • Resolving microvilli structure
  • Phase separation visualization
  • Membranal dynamics
 Angew. Chem., Int. Ed., 2022, 61, e202211540
JACS, 2019, 141, 14491
Autophagosomes
  • LC3 (SNAP/Halo-tag)
 22-25 (53.2)
  • Resolving autophagy process
 Anal. Chem., 2022, 94, 15057
Glycans
  • Bioorthogonal (metabolic labeling)
 18.2 (84.3, Cyto)
30.5 (124.6, PM)
  • Resolving sialylation bio-machinery
 Anal. Chem., 2021, 93, 7833

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